Centrifugal micro-channel array droplet generation for highly parallel digital PCR.

Supplementary Information Centrifugal Micro-Channel Array Droplet Generation for Highly Parallel Digital PCR

Agarose Droplet Microfluidics for Highly Parallel and Efficient Emulsion Pcr

An agarose droplet method was developed for highly parallel and efficient single copy emulsion PCR. By capitalizing on the unique thermal-sensitive sol-gel switching property of agarose, PCR is efficiently performed in agarose solution droplets, which is gelated to agarose beads after PCR to capture amplicons to maintain the monoclonality of each droplet. This method allows high throughput gene...

Controllable Active Micro Droplets Merging Device Using Horizontal Pneumatic Micro Valves

We present an active droplet merging device, which can merge various sizes of micro droplets in different numbers by using pneumatically controlled horizontal PDMS microvalves. The merging part consists of a main and side channels separated by a pillar array. The pillar array structure is contained within a microfuidic channel. The function of the pillar array provides a bypass path to the cont...

A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing.

We developed and characterized a novel picoliter droplet-in-oil array generated by a double-inkjet printing method on a uniform hydrophobic silicon chip specifically designed for quantitative polymerase chain reaction (qPCR) analysis. Double-inkjet printing was proposed to efficiently address the evaporation issues of picoliter droplets during array generation on a planar substrate without the ...

Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA.

Aqueous microdroplets provide miniaturized reaction compartments for numerous chemical, biochemical or pharmaceutical applications. We introduce centrifugal step emulsification for the fast and easy production of monodisperse droplets. Homogenous droplets with pre-selectable diameters in a range from 120 μm to 170 μm were generated with coefficients of variation of 2-4% and zero run-in time or ...